The Ames Site Office (AMSO) is an organization within the U.S. Department of Energy's Office of Science with responsibility to oversee and manage the Management and Operating (M&O) contract for the Ames National Laboratory in Ames, Iowa.

Ames National Laboratory is one of ten Office of Science Laboratories and is a single program laboratory with a primary mission on delivering breakthrough science and technology in the area of Basic Energy Sciences. The AMSO is responsible for program implementation, acquisition management, and laboratory stewardship at Ames National Laboratory. Through its oversight role, the AMSO is affecting the safe and efficient operation of Ames National Laboratory while enabling the pursuit of visionary research and development in support of DOE's missions.